Getting Started: A PDF with a quick overview of the FlowJo basics, click here.

Full Tutorials:

Basic Tutorial [- Demo data -]

• A basic titration experiment comprised of three parts: the example data set, a guided tour PDF file and completed workspace files for comparison. This tutorial will give you a start to finish look at FlowJo's basic functionality and mechanics.

Advanced Tutorial [ - Demo data -]

• A complex immunophenotyping experiment to illustrate handling complex data sets. This tutorial will highlight FlowJo's use of hierarchical gating, grouping and iterated batching. Like the Basic Tutorial, this contains example data sets, a guided tour PDF and completed workspaces for comparison.

Platform Demos and Complex Data Sets and Tech Notes:

Compensation [- Demo data - Tech Note ]

• This data set provides an introduction to performing compensation using FlowJo. For more information on FlowJo's Compensation platform, click here For Mac - For Windows .

Cell Cycle [-Demo Data - Tech Note ]

• This simple example illustrates how to perform Cell Cycle analysis. This data set is comprised of five files, collected at various points after synchronization of a cell line. Tree Star thanks Tom Delohery for kindly providing this demonstration data set. For more information on FlowJo's Cell Cycle platform, click here For Mac - For Windows

Proliferation [ - Zip data - 315 Kb]

• These samples were stained with CFSE, an intracellular dye used to measure cell proliferation, and then cultured for three days. With each cell division, the CFSE is divided between the two daughter cells. Therefore the amount of CFSE fluorescence shows the number of divisions any given cell has undergone. For more information on FlowJo's Proliferation platform, click here For Mac - For Windows

Kinetics [ -Demo Data - Tech Note ]

• The example data set demonstrates several features of the sophisticated kinetics platform that FlowJo provides. Cells were loaded with Indo-1. Collection for 30 seconds was performed to establish the baseline calcium concentration (related to the ratio of the Indo-1 fluorescences); then anti-CD3 was added. Within about 1 minute cells begin to respond by fluxing calcium; the Indo-1 emission ratio changes substantially over time. For more information on FlowJo's Kinetics platform, click here For Mac - For Windows

Calibration [-Demo Data ]

• This example illustrates how you can calibrate a parameter based on a standardized set of beads. For more information on FlowJo's Calibration platform, click here For Mac - For Windows

Full Immunophenotyping [-Demo Data ]

• The Full Immunophenotyping data set serves as an example of how a large experiment is analyzed and how the analyses are organized by FlowJo. The analyses depend largely on the "Group"-based operations in FlowJo, where entire sets of samples (i.e., all tubes with the same stain combination) are gated identically. For more information on Groups, click here For Mac - For Windows

Complex Analysis [-Demo Data ]

• There is only a single data file in this example. It is a collection of 300,000 events of PBMC stained with 8 different antibody conjugates. The reagents used in this experiment are: Cascade Blue anti-CD3, FITC anti-CD11a, PE anti-TcR gd, Cy5PE anti-CD45RA, Cy7PE anti-CD62L, Texas Red anti-CD4, APC anti-CD57, and Cy7APC anti-CD8. The goal in this stain was to identify fine T cell subsets.

11 Color Demonstration [ -Demo Data ]

• The PBMC sample in this collection was stained with 11 different antibodies: FITC anti-CD28, PE anti-CD49f, Cy5PE anti-CD11a, Alexa495 anti-CD62L, APC anti-CD45RO, Cy7APC anti-CD57, Cy7PE anti-CD4, Cascade Blue anti-CD45RA, Cascade Yellow anti-CD3, Cy5.5PE anti-CD27, and Cy5.5APC anti-CD8. The goal in this stain was to identify fine T cell subsets.

Large Data Files [ -Demo Data ]

• A single PBMC sample was stained with antibodies to CD3, CD4, and CD8. The sample was collect four times, with increasing numbers of events: 1000, 10,000, 100,000, and 1 million. Using these data files, you can explore how different types of displays can be dramatically different even though essentially the same data is being viewed.

To view or print the PDF tutorials and tech notes, you will need Adobe Acrobat Reader (available free from Adobe).

You may wish to have a printed copy of the FlowJo manual. Choose your platform, Mac or PC, and print the PDF file from your browser.
Printable Manual for FlowJo Macintosh
Printable Manual for FlowJo PC

We will send you a free printed copy upon request, if you call 1-800-366-6045, or send us e-mail.